Part:BBa_K5466006

SUC2 Signal Peptide. Saccharomyces cerevisiae codon optimized

Signal peptide consisting on the 19 first amino acids from Saccharomyces cerevisiae SUC2 invertase. Attachment to the N-terminal end of a protein can efficiently drive translocation across the plasma membrane.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

SUC2

SUC2 is an extracellularly-secreted invertase, an enzyme that hydrolyzes sucrose into glucose and fructose. The SUC2 signal peptide has been successfully used to secrete proteins or anchor them to the plasma membrane.

References

Durmusoglu, D., Al’Abri, I., Li, Z., Williams, T. I., Collins, L. B., Martínez, J. L., & Crook, N. (2023). Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach. Microbial Cell Factories, 22(1). https://doi.org/10.1186/s12934-023-02117-y